To investigate current status of the cultural literacy level of Traditional Chinese Medicine (TCM) health among college students majoring in medical and healthcare in the Zhuhai and Macau, the results of the survey can provide evidence for the government to formulate appropriate policies, and promote the development of Chinese medicine talents in the Guangdong-Hong Kong-Macau Greater Bay Area. Stratified cluster sampling was used to sample college students, and the data were analyzed by Χ²-test test and Logistic regression. A total of 964 questionnaires and 927 valid questionnaires were obtained. The overall level of health literacy of the sample population was 31.1%, the five-dimensional literacy level, from high to low, is healthy lifestyle literacy, cultural knowledge literacy, basic concept literacy, information comprehension ability, public appropriate methods literacy. There were significant differences between the literacy level of the students and the analysis results of their domicile, grade and major (P <0.05). The overall level of health cultural literacy among college students in Zhuhai and Macau is higher than that of national residents, but the scores in all dimensions are uneven. Domicile, grade and major are the factors that affect the level of dimensions of literacy. The colleges in Zhuhai and Macau should emphasize the importance of TCM health cultural literacy education and further improve the level of TCM health cultural literacy of students in Guangdong-Hong Kong-Macau Greater Bay Area.