Yu Zhenyi, Guo Wanyi, Hu Shaomin, Huang Jinxia, Shu Yongkang, Yu Lili
To verify that urinastatin (UTI) achieves anti-inflammatory effects by inhibiting membrane-bound prostaglandin E2 synthase-1 (mPGES-1) by using the carrageenan-induced foot swelling model. The efficacy of acute inflammation and found that UTI has no adverse cardiovascular effects. 40 SD rats were randomly divided into control group (n=10), model group (n=10), ulinastatin treatment group (n=10), and celecoxib treatment group (n=10). Carrageenan was used to cause paw swelling in rats, and was administered at the 0th hour of modeling. The UTI group was injected into the tail vein, and the control group and the model group were injected into the tail vein with the same volume of normal saline. The foot volume, plasma interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) of the four groups of animals at 0h, 1h, 2h, 3h, 4h, and 5h after modeling , Monocyte chemotactic protein-1 (MCP-1), Creatine kinase isoenzymes (CK-MB), and B-type natriuretic peptide (BNP) concentrations level, mPGES-1 and cyclooxygenase-2 (COX-2) gene expression levels in the foot.The foot volume of the model group, UTI treatment group and celecoxib treatment group increased from the 2nd hour after modeling, while the foot volume of the UTI and celecoxib treatment groups began to decrease significantly from the 3rd hour after modeling. Compared with the control group, the foot volume of the model group increased significantly (P<0.05), and the plasma levels of IL-6, TNF-α, and MCP-1 were significantly higher than those of the control group (P<0.05). The levels of plasma IL-6, TNF-α, and MCP-1 in the UTI treatment group and celecoxib treatment group were significantly lower than those in the model group (P<0.05). The plasma levels of CK-MB and BNP in the UTI treatment group were no different from those in the control group. Difference (P>0.05), while CK-MB in the celecoxib treatment group increased significantly (P<0.05). The mPGES-1 gene expression level in the feet of the UTI and celecoxib treatment groups was significantly lower than that of the model group (P<0.05), while the COX-2 gene expression level of the UTI treatment group was not significantly different from the model group (P>0.05).Ulinastatin is likely to achieve anti-inflammatory effects and reduce cardiovascular damage by specifically targeting the expression of mPGES-1 gene.